r/CannabisTissueCulture • u/lurktronic • 5d ago
No rooting - is my recipe off?
I didn't get rooting in this medium after 2-3 weeks (although the charcoal is making it hard to evaluate). Any thoughts? would it be ok to omit the Charcoal? My methods worked for stage 1 and stage 2. The K-IBA itself is the one thing that hasn't been tested in another batch.
Distilled / RO Water ~800 mL (to start, fill to 1L)
DKW Basal Powder (HALF STRENGTH) 2.66 g
Sucrose (White Table Sugar) 20 g
PPM™ (Biocide) 1.0 mL
K-IBA Stock (1" mg/mL" ")" 1.00 mL
Activated Charcoal 1.00 g
Target pH Range 5.7 – 5.8
Gellan Gum (Add LAST) 2.50 g